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KMID : 0363620080290020182
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Journal of Korean Oriental Medicine
2008 Volume.29 No. 2 p.182 ~ p.192
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Antimicrobial Effect on the Periodontal Pathogens and Anti-inflammatory Effect of Eriobotryae Folium
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Jeong Mi-Young
Kim Young-Hong
Lee Na-Kyung
Lee Jin-Yong
Herr Yeek
Lee Je-Hyun
Lim Sabina
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Abstract
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Objective: The purpose of this study was to evaluate on the antimicrobial effect on the periodontal pathogens and
anti-inflammatory effect of Eriobotryae folium. Eriobotryae folium are constituent herbs of Gagamgamroum, which has been used for a long time in oriental medicine as a herbal medicine for treating halitosis and toothache.
Method: Eriobotryae folium was prepared by extracting medicinal herb with water. We investigated antimicrobial
activity by the minimum inhibitory concentration (MIC) test. We also investigated inhibition of IL-1β-induced collagenase (mmp-1), stromelysin-1 (mmp-3), interleukin-6 gene expression in human gingival fibroblasts using RTPCR analysis.
Result: The antimicrobial effects of Eriobotryae folium was evaluated with MIC against periodontopathogens;
Porphyromonas gingivalis 2561, W50, A7A1-28, 9-14K-1, Prevotella intermedia 28, and Actinobacillus actinomycetemcomitans Y4. MICs of Eriobotryae folium were 1.25 / , 2.5 / , 0.625 / , 1.25 / , 10 / and 10 / . The anti-inflammatory effect of Eriobotryae folium was evaluated with influence of herbs on the IL-1β -induced expression of mmp-1, mmp-3, and interleukin-6. IL-1β increased mmp-1, mmp-3, and interleukin-6 mRNA levels. Eriobotryae folium significantly inhibited IL-1β-induced mmp-1, mmp-3, and interleukin-6 gene expressions in a dose-dependent manner.
Conclusion: These results suggested that Eriobotryae folium might reduce the excessive proteolytic capacity of the gingival fibroblast during inflammation and could be developed as a new drug for periodontitis.
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KEYWORD
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Periodontitis, Eriobotryae folium, collagenase (mmp-1), stromelysin-1 (mmp-3), interleukin-6
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